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미니돼지 정액 동결 시 Methyl-Beta-Cyclodextrin(MBCD)이 냉각 충격과 막 콜레스테롤량에 미치는 영향

Effects of Methyl-Beta-Cyclodextrin (MBCD) on Cold Shock and Membrane Cholesterol Quantity during the Freezing Process of Miniature Pig Spermatozoa

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  • URLhttps://db.koreascholar.com/Article/Detail/190979
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

This study was undertaken to find out the effect of methyl-beta-cyclodextrin (MBCD) on cold shock and membrane cholesterol quantity of sperm during the freezing process in miniature pigs. For this study, semen ejaculated from PWG M-type miniature pig was diluted that freezing solution (with egg yolk group) and m-Modena B (without egg yolk group) treated with 0, 1, 5, 10 and 20 mM MBCD before freezing process. The diluted semen was monitored sperm ability at room temperature, after cooled until 5℃ and after forzen-thawed for cold shock test of spermatozoa. Also, membrane cholesterol of sperm was extracted by folch solution at the same time. sperm ability was assessed for viability and acrosomal status. The membrane cholesterol quantity was measured by thin-layer chromatography (TLC) method. The result, viability and acrosome integrity in semen diluted without egg yolk groups were decreased at all temperature range by increasing of MBCD concentration. In particular, sperm of egg yolk group was showed that significantly higher viability and lower acrosome damage when treated with 5 mM MBCD (p<0.05). The results of TLC experiment, cholesterol amounts were increased with MBCD cocentration in egg yolk, and decreased with MBCD concentration in m-Modena B. In cryopreservation efficiency, there was no significant difference at viability, and acrosomal state of sperm in 5 mM MBCD concentration was significantly lower in acrosome damage than other groups (p<0.05). Therefore, the addition MBCD in egg yolk was protected spermatozoa from cold shock injury. This protective effect of MBCD may be due to addition of sperm membrane cholesterol.

목차
ABSTRACT   서론   재료 및 방법    정액의 준비    정액 동결 과정    동결 정액 융해 및 성상 검사    막 콜레스테롤 양 측정    실험 설계   결과    LEY 동결보존액과 Modena B에 MBCD 첨가 시 정자의 막 콜레스테롤의 변화    LEY 동결보존액과 Modena B에 MBCD를 첨가하여 냉각충격을 주었을 시 정자의 생존율과 첨체 상태의 변화    LEY 동결보존액에 각 농도(0, 1, 5, 10, 20 mM)의 MBCD를첨가하였을 시 동결 효율성   고찰   인용문헌
저자
  • 이성영(강원대학교 동물생명과학대학) | Sung-Young Lee
  • 이용승(강원대학교 동물생명과학대학) | Yong-Seung Lee
  • 정희태(강원대학교 수의과대학) | Hee-Tae Cheong
  • 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang
  • 박춘근(강원대학교 동물생명과학대학) | Choon-Keun Park Corresponding author