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In Vitro Development of Primordial Germ Cells Nuclear Transfer Porcine Embryos

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  • URLhttps://db.koreascholar.com/Article/Detail/191585
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발생공학 국제심포지엄 및 학술대회 (International Symposium on Developmental Biotechnology)
한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

Unstable Epigenetic reprogramming was DNA methylation, imprinting, RNA silencing, co-valent modifications of histones and remodelling by other chromatin-associated complexes. After fusion with an enucleated oocyte, a differentiated somatic cell can restructure its genetic program and acquire totipotent characteristics. However, these cases happen only with low frequency. primordial germ cells (PGC) was effectively remove of epigenetic modifications in the genetic totipotency which is necessary for the development. The present study was in vitro development of reconstruct PGC NT embryos compared with somatic cell NT embryos. The rate of cleavage did not differ between NT embryos from PGC and somatic cells (87.26 vs 91.36%). However, the rate of development to the blastocyst stage was significantly higher in PGC cell NT than somatic cell NT (31.03 vs 19.27%). PGC from a slightly younger stage of development have succeed to promote normal development of recipient eggs. This difference in results between germ cell and somatic cell nuclear transfers could only be a reflection of intimate differences in their reprograming. These results suggest that PGC NT embryos are significantly higher for the in vitro development. Furthermore, These study may represent an approach towards achieving better production of transgenic animal.

저자
  • Byoung-Chul Yang(National Institute of Animal Science, RDA)
  • Jin-Hee An(National Institute of Animal Science, RDA, College of animal life science, Kangwon National University)
  • Kyung-Won Kim(National Institute of Animal Science, RDA)
  • Hak-Jae Chung(National Institute of Animal Science, RDA)
  • Hee Kyung Jung(National Institute of Animal Science, RDA)
  • Hyun-Mi Kim(National Institute of Animal Science, RDA)
  • You-Mi Cho(National Institute of Animal Science, RDA)
  • Choon-Keun Park(College of animal life science, Kangwon National University)
  • Jin-Ki Park(National Institute of Animal Science, RDA)