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Establishment and X-chromosome Inactivation Status in Female of Porcine EpiSC-like Embryonic Stem Cells and Induced Pluripotent Stem Cells
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/191653
Several studies have been conducted with the aim of establishing embryonic stem cell lines from porcine embryos. However, most researchers to date have found it difficult to maintain an ES-like state in derived cell lines, with the cells showing a strong tendency to differentiate into an epithelial or EpiSC-like state. We have also been able to derive cell lines of an EpiSC-like state and a differentiated non-ES-like state from porcine embryos of various origins, including invitro fertilized(IVF), in vivo derived, IVF aggregated and parthenogenetic embryos. In addition, we have generated induced pluripotent stem cells(piPSCs) via plasmid transfection of reprogramming factors (Oct4, Sox2, Klf4 and c-Myc) into porcine fibroblast cells. X chromosome inactivation (XCI) have recently been addressed as a hallmark to determine whether pluripotent cell is naïve or primed state. In this study, we could confirm the X chromosome inactivation status in female cell lines as well as marker expression, pluripotency and of our Epi- SC-like pESC lines along with our piPSC line. All of our cell lines showed AP activity and expressions of the genes Oct4, Sox2, Nanog, Rex, TDGF1, bFGF, FGFR1, FGFR2, Nodal and Activin-A involved in pluripotency and signaling pathways, XCI in female cell lines, in vitro differentiation potential and a normal karyotype, thus displaying similarities to epiblast stem cells or hES cells. Therefore, it may be inferred that, as a non-permissive species, the porcine species undergoes reprogramming into a primed state during the establishment of pluripotent stem cell lines.
