The aim of this study was to evaluate the effects of orvus es paste(OEP) on the sperm characteristics during freezing in boar semen. Semen quality was evaluated the motility, membrane integrity, mitochondria function, acrosome status, viability and abnormality. Boar semen were frozen until 5℃ for 2 hours using cell freezer and making the straws, and then freezing by lowing the straws into styrofoam box on the 8cm above the LN2 and plunged into LN2 for cryopreservation. In different concentration of OEP (0, 0.25, 0.5 and 1.0%) into cryo-extender, sperm motility, membrane integrity, acrosome status, viability and mitochondria function were significantly higher in 0.5% OEP than those of any other groups, but sperm abnormality were highest in 1.0% OEP group among all treatment groups (P<0.05). In the relationships of the evaluation methods for sperm viability, CBB vs membrane integrity, CBB vs HO/PI and CBB vs mitocondria function were positively correlated (0.67~0.92). Among the evaluation methods, CBB vs membrane integrity, CBB vs HO/PI and CBB vs mitocondria function were significantly correlated (P<0.001). These results of this study indicate that supplementation of 0.5% OEP in boar semen cryo-extender can improve the semen quality.

ABSTRACT   Ⅰ. 서론   Ⅱ. 재료 및 방법    1. 정액채취    2. 돼지 정액의 동결 방법    3. 동결 정액의 융해 및 체외 배양    4. 정액의 성상검사    5. 통계처리   Ⅲ. 결과 및 고찰    1. 돼지 정액 동결시 OEP의 첨가가 융해 후 정액 특성에 미치는 효과    2. 돼지 정액 동결시 OEP의 첨가가 융해 후 정자의 원형질막의 기능성에 미치는 효과    3. 돼지 정액 동결시 OEP 첨가가 정자의 첨체에 미치는 효과    4. 돼지 정액 동결시 OEP 첨가가 정자의 생존율에 미치는 효과    5. 돼지 정액 동결 시 OEP 첨가가 mitocondria의 기능에 미치는 효과    7. 정자 생존력에 대한 측정방법간의 상관관계   Ⅳ. 요약   Ⅴ. 인용문헌

• 장현용(강원대학교 동물생명과학대학) | Hyun-Yong Jang
• 김영한(강원대학교 동물생명과학대학) | Young-Han Kim
• 박춘근(강원대학교 동물생명과학대학) | Choon-Keun Park
• 김종택(강원대학교 수의학부대학) | Jong-Tak Kim
• 정희태(강원대학교 수의학부대학) | Hee-Tae Cheong
• 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang Corresponding Author