This study was conducted to investigate the effect of periods of sperm preincubation, concentrations and storage periods of miniature pig sperm on in vitro penetration of porcine follicular oocytes. High concentration (1×105, 2.5×105, 5×105, 1×106, 5×106 and 1×107) did support sperm penetration than low concentrations (P<0.05). However, polyspermic oocyte rates were increased with high concentrations of sperm. On the other hand, sperm preincubated during 1, 2 or 5h could be penetrated than sperm preincubated during 0, 3 or 4h (P<0.05). When sperm were storaged with different periods, in vitro pentration rates were significantly higher 0～2 days than 3～4 days of sperm storage (p<0.05). These results indicate that sperm treatment factors can effect in vitro penetration in miniature pig.

ABSTRACT   Ⅰ. 서론   Ⅱ. 재료 및 방법    1. 난자의 준비    2. 난자의 체외성숙    3. 정자의 준비    4. 체외수정 및 체외배양    5. 실험설계    6. 난자의 관찰    7. 통계처리   Ⅲ. 결과   Ⅳ. 고찰   Ⅴ. 요약   감사의 글   Ⅵ. 인용문헌

• 안신혜(강원대학교 동물생명과학대학) | Shin-Hye Ahn
• 이유미(강원대학교 수의학부대학) | Yu-Mi Lee
• 정희태(강원대학교 수의학부대학) | Hee-Tae Cheong
• 양부근(강원대학교 동물생명과학대학) | Hee-Tae Cheong
• 이동석(강원대학교 동물생명과학대학) | Dong-Seok Lee
• 박춘근(강원대학교 동물생명과학대학) | Choon-Keun Park Corresponding Author