The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.

ABSTRACT   I. 서론   II. 재료 및 방법    1. 시료 준비 및 시약    2. 해양성 광천수의 삼투압 측정    3. 정액 채취 및 운반    4. 정액의 보존    5. 정액의 성상평가   III. 결과 및 고찰    1. 해양성 광천수의 삼투압 측정    2. 미니돼지 정액 보존 시 해양성 광천수가 정자의 생존율에 미치는 효과    3. 첨체반응과 수정능 획득 정자    4. HOST 양성반응율    5. 정자의 기형율 평가   IV. 요약   사사   V. 인용문헌

• 이상희(강원대학교 동물생명과학대학) | Sang-Hee Lee
• 이재흥(강원대학교 동물생명과학대학) | Jae-Heung Lee
• 정희태(강원대학교 수의과대학) | Hee-Tae Cheong
• 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang.
• 김효미(금진생명과학) | Hyo-Mi Kim
• 이샛별(금진생명과학) | Saet-Byeol Lee
• 권순성(금진생명과학) | Soon-sung Kwon
• 최선강(강릉과학산업진흥원) | Seon-Kang Choi
• 심재만(강릉과학산업진흥원) | Jae-man Sim
• 김정득(금진생명과학) | Jung-Duk Kim
• 박춘근(강원대학교 동물생명과학대학) | choon-Keun Park Corresponding Author