Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    Cell Lines    Feeder Preparation and Embryonic Stem (ES) Cell Culture    Feeder Free Culture of Embryonic Stem (ES) Cell    Induce Differentiation by Withdrawal of LIF   RESULTS    Phenotype of ES Cell and Feeder    The ES Cell Culture on the Feeder Free Condition    The ES Cell Induce Differentiation on the Feeder FreeCondition   DISCUSSION   REFERENCES

• Myoung Ok Kim(School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea)
• Zae Young Ryoo(School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea) Corresponding author