To prepare calcium-binding peptides as calcium supplement, barley proteins were hydrolyzed using Flavourzyme for 18 h and the hydrolysates were ultra-filtered under 3 kDa as a molecular weight. The resultant filtered peptides were fractionated using ion exchange and normal-phase high performance liquid chromatography. Then each fraction that was obtained was determined for its calcium-binding activity to isolate the calcium-binding peptides. As a result, the highest calcium-binding peptide fraction was obtained, and the results suggest that barley protein hydrolysates can be used as a calcium supplement.

• 이지혜(충남대학교 농업생명과학대학 식품공학과) | Ji Hye Lee
• 최동원(충남대학교 농업생명과학대학 식품공학과) | Dong Won Choi
• 송경빈(충남대학교 농업생명과학대학 식품공학과) | Kyung Bin Song Corresponding author