In the fishing process on the sea eel pots, the most dangerous and difficult labor is manually separating process of several thousand pots from main line with high speed hauling operation. In this study, the automatic separating system of the pot from main line is developed. This system is composed with the clip connected to the pot by branch line, the keeper attached to the main line by pressing and the separator separating the clip and the keeper. In the experiment, static and dynamic characteristics of the system are investigated. From the considerations on the experimental results, it is ascertained that the automatic separating system of the pot from main line proposed in this study has good operating performance.