This study was performed to have an effect on control the growth of algae such as "Actinastrum fluviatile" which occurs frequently in the aquaculture tank using by a cold plasma, and it also would be expected to promote the growth of water plants due to active element causing OH radicals in the water. In addition, it was verified on the death rate as 99.9% on the scale protrusion of "Aeromonas hydrophila" as well as E. coli, especially, under extreme conditions more than 100 million pathogenic bacterium in the aqua pet tank, the ornamental fish had to act in a safe and healthy environment at over 98% death rate within 48 hours. It has been proven to give no affect on aerobic bacteria that exist in the filter or soil because there was no residual toxicity in the water tank. As the results, it will help to develop and apply on the sterilization device in other industries as well as aquarium organisms due to adopted energy-saving algorithm and reliability in use.