Oil dispersants using polyoxyethylene monooleate, polyoxyethylene oleylether, and poly(oxypropylene-oxyethylene)glycol block copolymer were prepared, and oil dispersant efficiency was measured using vertical shaking flask method to 4 kinds of Bunker B oil with different physical properties by appling the prepared dispersants. Although the dispersant efficiency was differed according to the differences of physical properties of Bunker B oil, the dispersant prepared using polyoxyethylene oleylether was the most effective to disperse the oil into water. The impurities like surfur contained in sample oil have to be removed by filteration to obtain the correct degree of absorption using UV spectrophotometer.