Metachromasy of Metylene Blue and Thionine by Analytical Fluorescence Spectroscopy
Metachromatic properties of admixture of methylene blue(MB) and thionine in aqueous solution has been studied by fluorescence spectroscopy. In spite of nonfluorescence character has been MB itself, mixing MB to monomeric concentration of thionine, new coaggregation band has been formed in shorter wave length than fluorescence of thionine because of MB was redistributed to thionine aggregate. It suggested that coaggregate of MB and thionine were more tightly formed than the each dye aggregate.