In spite of various applications of chitin derivatives from waste marine sources, commercial use of chitin has been limited due to resistance to chemicals and the absense of proper solvents. We prepared chitin through decalcification, bleaching and deproteination from protunus trituberculatus shells by the application of Hackman's method. Structural and chemical properties of chitin were investigated to have proper specific surface area and particle size by IR, BET and PSA. The amount of absorbed water of chitin reached equilibrium by stirring about 15 minutes. The amount of absored water of the prepared chitin were large than the commercial chitin. When prepared chitin tested on dyeing wastewater, they showed better treatment efficiency in COD, suspended solid, and color tests than the commercial chitin. The adsorption capacity increased with decreasing particle size for the prepared chitin. Treatment efficiency for color was increased as the sitirring rate increased. Results show the possibility of the prepared chitin from waste marine sources as a treatment system for dyeing wastewater.