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Lilium longiflorum × L. X elegans 의 자방배양에 의해 얻어진 잡종 F1의 핵형 , 감수분열 및 lsozyme에 대한 연구 KCI 등재

Study of Karyotype , Meiosis and Isozyme of Hybrid from cross Lilium longiflorum x L. X elegans

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한국자원식물학회지 (Korean journal of plant resources)
한국자원식물학회 (The Plant Resources Society Of Korea)
초록

Hybries which was made up by chromosome of L. longiflorum and L. x elegans, using root-tip individual which was obtained through ovary slice culture, and root-tip of these parents, with hoirugen staining, gimsa staining and Q-H staining inaccordance with the location and the existence of secondary construction which waslocating near short arm centromere of No, 1,2,6,9. In metaphase of meiosis ofhybrid which was made up by univalent from 2 individuals to 10 individuals wasobserved, and nuclear plate which was having abnormal type's synthesis amounted to91% of all cells whieh were observed. This result showed the fact that someobstacle arose annormal progress of the divission after that time. 63% of the cellshad micronucleus from 1 individlial to 4 individuals in tetrad phase of meiosisdivision. The peroxidase and α -estelase zymogram phenotypes of parents andhybrids were determined using agarlose IEF gel. Crosses were performed betweenparents bearing dissimilar allelomorphs in orther to discern the genetic control ofthe resolved enzymes. Genetic variation of hybrids were detected at all but 2 plant progenies.

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