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Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata

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  • URLhttps://db.koreascholar.com/Article/Detail/259438
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한국자원식물학회 (The Plant Resources Society Of Korea)
초록

To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.

저자
  • Kang Lee
  • Jun-Gyo In
  • Chang-Yeon Yu
  • Song-Joong Yun
  • Byung-Hoon Min
  • Yeong-Deok Rho
  • Moo-Sung Kim
  • Deok-Chun Yang