This study investigated physiological characteristics and genetic relationship of 30 strains of Lentinula edodes, collected from the Europe, Asia, North America and preserved in the Forest Mushroom Research Institute(FMRI). In physiological characteristics, Papua New Guinea strain was excellent mycelium growth in 25℃ for 7 days on PDA media. For all strains, the optimal temperature for mycelial growth, their tunicate and color of hypha were observed. It surveyed their mycelial growth on oak sawdust media in test tube and independence with inter-strains and cultivar developed in FMRI by strain's anastomosis culture. It was carried by RAPD using operon primers as molecular genetic methods, investigated genetic relationships among strains using UPGMA in NYSYSpc(2.1) according to the presence or absence of bands. <This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries>

• Sun-Gyu Choi(National Forestry Cooperatives Federation, Forest Mushroom Research Institute, Yeoju, Gyeonggi-Do, 469-803, Korea)
• Han-Gyu Ko( National Forestry Cooperatives Federation, Forest Mushroom Research Institute, Yeoju, Gyeonggi-Do, 469-803, Korea) | Han-Gyu Ko
• Jong-Hyun Noh( National Forestry Cooperatives Federation, Forest Mushroom Research Institute, Yeoju, Gyeonggi-Do, 469-803, Korea) | Jong-Hyun Noh
• Seon-Cheol Kim( National Forestry Cooperatives Federation, Forest Mushroom Research Institute, Yeoju, Gyeonggi-Do, 469-803, Korea) | Seon-Cheol Kim
• Chang-Hyun You( National Forestry Cooperatives Federation, Forest Mushroom Research Institute, Yeoju, Gyeonggi-Do, 469-803, Korea) | Chang-Hyun You
• Heung-Soo Park( National Forestry Cooperatives Federation, Forest Mushroom Research Institute, Yeoju, Gyeonggi-Do, 469-803, Korea) | Heung-Soo Park