The mannanase, cellulase and xylanase-producing thermophilic bacteria, designated EJ3, was isolated from fresh spent mushroom (Pleurotus eryngii) substrates taken from the DOJUN farm located in Keyongnam, Korea. The isolate EJ3 was facultatively anaerobic and grew at temperature ranging from 20℃ to 60℃ with an optimal temperature of 37℃. The DNA G+C content of the isolate EJ3 was 45 mlo%. The major fatty acids were anteiso-15:0 (38.9%), 17:0 (7.4%), and iso-15:0 (38.2%). The 16S rRNA gene sequence similarity between the isolate EJ3 and other Bacillus strains varied from 97% to 99%. In the phylogenetic analysis based on these sequences, the isolate EJ3 and Bacillus subtilis clustered within a group and separated from other species of Bacillus. Based on the physiological and molecular properties, the isolate EJ3 was classified within the genus Bacillus as Bacillus subtilis EJ3. The optimal growth condition of B. subtilis EJ3 were pH 6.0 and 55℃, respectively.

• Eun Ji Choi(Department of Pharmaceutical Engineering, Jinju National University)
• Yun Jin Cho( Department of Pharmaceutical Engineering, Jinju National University) | Yun Jin Cho
• Pyung Gyun Shin( Division of Mushroom Research, International Institute of Horticultural & Herhal Science, Suwon, 441-707) | Pyung Gyun Shin
• Young Bok Yoo( Division of Mushroom Research, International Institute of Horticultural & Herhal Science, Suwon, 441-707) | Young Bok Yoo
• Young Un Cho( Department of Pharmaceutical Engineering, Jinju National University) | Young Un Cho
• Hong Chul Kim( Department of Pharmaceutical Engineering, Jinju National University) | Hong Chul Kim
• Sang Wan Gal( Department of Pharmaceutical Engineering, Jinju National University) | Sang Wan Gal
• Yea Hwang Moon( Department of Animal Science & Biotechnology, Jinju National University) | Yea Hwang Moon
• Soo Jeong Cho( Department of Pharmaceutical Engineering, Jinju National University) | Soo Jeong Cho