The Lentinula edodes is the third most widely produced mushroom in the world. It is a rich source of proteins, fats, carbohydrates, fiber, vitamins, minerals and β -glucan. The aim of the this study was to evaluate the physicochemical characteristics changes such as proximate composition, minerals, and contents of β -glucan according to the different product area. And we evaluated the antioxidant properties of L. edodes. Also, we determined the standardization of manufacture process for commercial products using L. edodes. Eleven L. edodes were cultivated in oak, and samples were collected by different product area. And the L. edodes cultivated in sawdust have been producted in Jangheung. All samples are shade dried and those dried samples were used for this test.