The aim of this study was to investigate the antitumor activity of solvent fractions from Auricularia auricula-judae 70% ethanol extract and confirmed the active components of dichloromethane fraction showing a potent antitumor activity than other fractions in the broncheoalveolar and gastric cancer cells. The solvent fractions of Auricularia auricula-judae extract, inhibited the growth proliferation of tumor cells in dose-dependent manner. The principle components of dichloromethane fraction were 5,11,17,23-tetrakis (1,1-dimethyl)-28-methoxypentacyclo [19.3.1.1 (3,7).1 (… (65.85%) and diazane (6.17%). The antitumor active components, diazane and gibberellic acid (GA3) were identified in this fraction by GC-MS analysis and lower antitumor activities than dichloromethane fraction. The unknown components of dichloromethane fraction were responsible for its cytotoxic effects on tumor cells. Based on IC50 value, gibberellic acid was little cytotoxic itself. According to PCR amplification, the apoptosis of tumor cells were induced by the down-regulation of Bcl-2 and over-expression of P53 on the presence of solvent fractions, diazane and gibberellic acid. Thus, these findings suggest that the dichloromethane might be used as functional feed additive that suppress the tumor growth in the body than other solvent fractions of Auricularia auricula-judae extracts.

• Md. Ahsanur Reza(College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea)
• Joong-su Lee( College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea) | Joong-su Lee
• Seung-jin Lee( College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea) | Seung-jin Lee
• Woo-Sik Jo( Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-701, Republic of Korea) | Woo-Sik Jo
• Seung-chun Park( College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea) | Seung-chun Park