Cosmetic activities of the leaves of Curcuma longa L. were compared according to different drying methods, to expand the use of the by-products of Curcuma longa L. The highest extraction yield of 29.4% was obtained from vacuum drying process (VD), whose value was very close to 29.2% from freeze drying process (FD). Relatively lower extraction yield were observed as 24.5% and 25.3% from the raw leaf (RL) as control and hot air drying process (HD). The cytotoxicity was observed lower FD and VD than RL and HD. It shows that cytotoxicity was closely related to cosmetic activities such as tyrosinase and melanin inhibition activities by showing much better cosmetic activities of the extracts from both FD and VD than those from the RL and HD. It was interesting that was differences of the cosmetic activities and cytotoxicity between FD and VD, which implies that VD method should be a more efficient process than FD method in terms of drying time and operation costs: 6 hours vs 24 hours and 3-5 times higher extraction costs in drying. It was observed that VD is more excellent dry method than others. This result could be utilized to effectively dry other soft plant biomass.

 ABSTRACT  서 언  재료 및 방법   1. 실험재료 및 시약   2. 시료 추출 조건   3. 세포주 및 세포 생육 배지   4. 세포 독성 측정   5. Tyrosinase 억제활성 측정   6. Clone M-3 세포로부터 Melanin 생성 저해율 측정   7. Hyaluronidase 활성 저해 효과 측정   8. Scanning Electron Microscope (SEM)을 통한 강황 잎조직 관찰   9. 통계처리  결과 및 고찰   1. 수율   2. 세포 독성 측정   3. Tyrosinase 억제활성 측정   4. Clone M-3 세포로부터 Melanin 생성 저해율 측정   5. Hyaluronidase 활성 저해 효과 측정   6. Scanning Electron Microscope (SEM)을 통한 강황 잎조직 관찰  감사의 글  REFERENCES

• 김남영(강원대학교 생물의소재공학과) | Kim, Nam Young
• 임혜원( 세바바이오텍) | 임혜원
• 이현용( 서원대학교 식품공학과) | 이현용