활성산소종(reactive oxygen species, ROS)인 glucose oxidase(GO)의 산화적 손상이 배양 C6glioma 세포에 미치는 영향 및 이에 대한 연꽃(Nelumbo nucifera GAERTN, NNG) 추출물의 항산화 효과를 세포생존율(cell viability)을 비롯한 DPPH-radical scavenging activity, lipid peroxidation에 의하여 분석하였다. 본 연구에서 H2O2는 배양 C6 glioma세포에 처리한 농도에 비례하여 유의한 세포생존율의 감소를 나타냈다. 한편, GO의 산화적 손상에 대한 NNG추출물의 방어효과를 조사하기 위하여 세포생존율을 조사한 결과 NNG 추출물은 GO의 산화적 손상으로 감소된 세포생존율을 유의하게 증가시켰다. 또한, NNG 추출물은 DPPH-radical scavenging activity의 분석에서 유의한 자유라디칼 소거능을 보였으며, 동시에 지질과산화에 대한 억제능을 보였다. 위의 결과로 부터 GO는 배양 C6 glioma세포에 고독성인 것으로 나타났으며 NNG추출물은 GO와 같은 ROS의 산화적 손상을 방어하는데 효과적인 것으로 나타났다.

This study was aimed to clarify the cytotoxic effect of reactive oxygen species (ROS), glucose oxidase(GO) on the cultured C6 glioma cells, cell viability by XTT assay was determined by colorimetric assay and also, the protective effect of Nelumbo nucifera GAERTN (NNG) extract on cultured C6 glioma cells. C6 glioma cells were grown in the media containning 10∼40mU/mL concentrations of GO for 6 hours, and cell viability, 1,1-diphenyl-2-picrylhydrazyl(DPPH)-radical scavenging activity and also lipid peroxidation by ferric thiocyanate (FTC) assay was assessed on cultured in these cultures. In this study, GO remarkably decreased cell viability in a dose dependent manner. The XTT90 and XTT50 values were determined at 10mU/mL and 40mU/mL of GO by the treatment of C6 glioma cells with GO. In the protective effect of NNG extract on the cytotoxicity induced by GO, NNG extract significantly increased cell viability decreased by the cytotoxicity of GO. NNG extract also showed DPPH-radical scaveng activity and the diminutive activity of lipid peroxidation. From these results, it is suggested that GO showed highly toxic effect on cultured C6 glioma cells and NNG extract such as flavonoids showed the protection on the cytotoxicity induced by GO in these cultures.

ABSTRACT
  Ⅰ. 서론
  Ⅱ. 재료 및 방법
  Ⅲ. 결과 및 고찰
  Ⅳ. 적요
  Ⅴ. 인용문헌

• 오용열(원광대학교 의과대학 산본병원) | Yong-Leol Oh