Masspropagation of Korea Green Native Ferns In Vitro for Developing Materials of Interior Landscape Architecture 4. The Growth of Ferns In Vivo, as Affected by Cultural Soil Media, pH, Ammonium Nitrate and Shading Rates
자생 상록양치류의 대량번식의 체계확립을 위해 기내배양 식물체의 순화기에 있어 순화기 용토종류와 용토내의 pH, 관비효과를 위해 ammonium nitrate 그리고 차광율을 조사한 결과는 다음과 같다. 자생상록 양치류의 순화기에 이식용토 종류를 달리하여 더부살이고사리 외 4종의 상록자생 양치류의 전엽체를 이식함에 있어 전반적으로 상토+마사+펄라이트(2:1:1) 조합에서 포자체의 형성과 생장이 양호하였다. 자생상록 양치류의 순화용토 내에 적합한 pH는 공시식물 종에 따라 적성 pH의 요구성이 pH 5.5~7.5로 다양하게 보였으며, 순화기에 차광 효과는 30%와 50% 차광에서 무차광구에 비해 포자체 형성과 생장이 증가하였다. 또한 순화기의 관비에 관한 효과는 ammonium nitrate 0.5 g/ℓ 첨가가 순화기에 효과적임을 알 수 있었다.
This study was carried out to establish the in vitro mass propagation system of korea green native ferns for developing materials of interior landscape architecture. This study was also performed to investigate the growth of ferns in vivo, as affected by cultural soil media, pH, ammonium nitrate and shading rates on the propagation of prothallus for several korea green native ferns.
The prothallus grown on soil in vivo were transferred into culture bottles filled with artificial soil wetted with Hoogland solution. The effects of soil composition were varied. The cultural media (compost:masa:perlite, 2:1:1) seemed to be the most suitable for the prothallus growth and shoot formation in Pteris cretica, Diplazium lanceum, Asplenium scolopendrium, except for Polystichum lepidocaulon. In the condition of acclimation stage, these results showed that the rate of shading in 30~50% were more effective for the prothallus growth and shoot formation. Althrough the plantlets grew at wide range of pH, cultural soil media supplemented with 0.5g/ℓ ammonium nitrate were occurred simultaneously.