논문 상세보기
pp.159-164
1. ‘Florex Gold’ 품종의 측아 기내배양에서 auxin류의 단용처리는 multiple shoot 발생을 유도하지 못했다. Callus 형성은 2,4-D 1.0 mg/L 처리구에서 63.3%로 가장 높았고, multiple shoot 형성은 cytokinin인 zeatin 1.0 mg/L 처리구에서 평균 8.1개로 가장 높게 나타났다.
2. ‘Florex Gold’의 증식 실험에서 callus 형성은 NAA 1.0 mg/L와 BA 1.0 mg/L이 혼용처리된 MS 배지에서 26(86.7%)개의 절편체에서 발생해 가장 높았다. 또한 동일한 배지 조건이 multiple shoot발생에도 가장 효과적이었고, 평균 16.9개(평균길이4.6 cm)의 신초가 발생하였다.
3. ‘Florex Gold’ 신초의 발근유도 실험에서 2,4-D 1.0 mg/L처리가 절편체당 평균 6.7(3.2 cm)개의 뿌리를 유도하여 ‘Florex Gold’ 신초의 발근 유도에 가장 적합한 것으로 나타난 반면 NAA는 발근유도에 부적합 한 것으로 나타났다.
Calla lily bulbs are prone to decaying disease above 28oC. Therefore, it is very difficult to produce or obtain a good commercial calla lily bulb at low price in temperate regions with hot summer. In such regions, calla lily seedlings from in-vitro micro-propagation can be used alternatively. In this study, we carried out experiments to establish appropriate in-vitro micro-propagation conditions of calla lily 'Florex Gold'. MS medium containing NAA 1.0 mg/L and BA 1.0 mg/L was optimal for both callus and multiple shoot formation. In the given MS media, 26 (86.7%) explants formed callus and all 30 explants produced multiple shoots with average of 16.9 shoots per explant. Obtained shoots were used for root induction on MS media supplemented with NAA, IAA, or 2,4-D respectively to determine an appropriate auxin for rooting. The best rooting was observed in MS media supplemented with 1.0 mg/L of 2,4-D, in which an average of 6.7 roots were generated per explant, indicating that 1.0 mg/L of 2,4-D is optimal for rooting of shoots derived from lateral buds of ‘Florex Gold’.
