Anti-inflammatory activity of the extracts of ginseng berry (GBE) was investigated through the evaluation of its inhibitory effect on the production of inflammatory meditator, nitric oxide(NO), tumor necrocis factor-alpha (TNF-α), interleukin-6 (IL-6) in LPS-induced RAW264.7 macrophage cells. GBE was fractionated using n-hexane, chloroform, ethylacetate, buthanol and H2O, sequentially. RAW264.7 cells were induced 100ng/mℓ of lipopolysaccharide (LPS) and treated with 0, 1.6, 8, 40 and 200μg/mℓ of GBE fractions. LPS-induced NO production on all of GBE fractions was inhibited with increasing added concentration of GBE fractions. Chloroform fraction of GBE was the most effective in inhibiting LPS-induced TNF-α production. Hexane, chloroform and H2O fractions of GBE exhibit strong inhibition LPS-induced IL-6 production. Especially, H2O fractions of GBE was the most effective in inhibiting LPD-induced IL-6 production without significant cytotoxicity in RAW264.7 cells, and reduced the activation of mitogen-activated protein kinases (MAPK) and IkB phosphorylation. These results indicate that H2O fractions of GBE exhibits strong anti-inflammatory effects by inhibition of NF-kB by inhibition of p-38 on MAPK and IkB phosphorylation.

ABSTRACT
 서 언
 재료 및 방법
 결과 및 고찰
 감사의 글
 REFERENCES

• 이가순(충남농업기술원 금산인삼약초시험장) | Lee, Ka Soon Corresponding author
• 김관후( 충남농업기술원 금산인삼약초시험장) | 김관후
• 성봉재( 충남농업기술원 금산인삼약초시험장) | 성봉재
• 김선익( 충남농업기술원 금산인삼약초시험장) | 김선익
• 한승호( 충남농업기술원 금산인삼약초시험장) | 한승호
• 이석수( 충남농업기술원 금산인삼약초시험장) | 이석수
• 양혜( 건양대학교 의과대학) | 양혜
• 유영춘( 건양대학교 의과대학) | 유영춘