Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was 2.68±0.21 - 5.87±2.43 in year 1 and was 3.24±0.37 - 9.05±0.16 in year 2. The stachyose content (g/kg) was 4.23±0.98 - 27.68±9.90 at year 1 and was 5.11±1.09 - 25.32±0.35 in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation (R2=0.1985*) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.

ABSTRACT
 재료 및 방법
  유전자원
  Raffinose 및 stachyose 함량 분석
 결과 및 고찰
  Raffinose와 stachyose의 함량변이
  Raffinose와 stachyose 함량의 년차간 변이
  Raffinose와 stachyose 함량의 상관
 적 요
 사 사
 인용문헌(REFERENCES)

• 성미경( 경상대학교 농학과, 생명과학연구원 ) | Mi Kyung Sung ( Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University, Jinju 660-701, Korea )
• 한성진( 경상대학교 농학과, 생명과학연구원 ) | Sung Jin Han ( Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University, Jinju 660-701, Korea )
• 서형진( 경상대학교 농학과, 생명과학연구원 ) | Hyung Jin Seo ( Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University, Jinju 660-701, Korea )
• 최상우( 경상대학교 농학과, 생명과학연구원 ) | Sang Woo Choi ( Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University, Jinju 660-701, Korea )
• 남상해( 경남과학기술대학교, 식품과학부 ) | Sang Hae Nam ( Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea )
• 정종일( 경상대학교 농학과, 생명과학연구원 ) | Jong Il Chung ( Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University, Jinju 660-701, Korea )