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국내 수수 종자의 용해도별 단백질 함량 변이 KCI 등재

Variation of Fractionated Protein Content by Solubility in Korean Local Sorghum Seed

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한국작물학회지 (Korean Journal of Crop Science)
한국작물학회 (Korean Society Of Crop Science)
초록

Sorghum seed is traditionally used as health supplements and the secondary food mixed with rice in Korea. While the research of reserve protein in sorghum seed have been carried out in many countries used as major food, much less is known about reserve proteins of Korean local sorghum seeds. To obtain protein characteristics in 20 Korean local sorghum seed, quantitative content of reserve protein was determined after fractionation by modified ‘Osbone’ method and α-kafirin of prolamin was determined by SDSPAGE. Mean albumin, globulin, prolamin and glutelin contents based on total seed protein content of 20 Korean local sorghum seed were 6.2%, 0.9%, 57.9% and 35.1%, respectively. Sorghum cultivar with high prolamin were ‘Whin-susu’, ‘Whin-Chalsusu’, ‘Whanggeum-Chalsusu’, and ‘Daepungshushu’. Sorghum cultivar with high α-kafirin were ‘Whin-susu’, ‘Geumsan-Chalsusu’, ‘Whin-Chalsusu’, and ‘Jangmok-susu’. Among the 20 varieties, ‘Whin-susu’ and ‘Whin-Chalsusu’ were selected as high α -kafirin and prolamin sorghum cultivar, which showed 64.5 and 71.9% of prolamin contents, respectively.

목차
ABSTRACT
 재료 및 방법
  시료
  기기 및 장비
  종자 저장단백질 추출
  단백질 정량
  프롤라민 단백질의 SDS-PAGE
 결과 및 고찰
  수수 종자단백질의 총 수용성 단백질 함량
  수수 종자의 용해도에 의한 분획 단백질 함량
  수수종자의 저장단백질 프롤라민 단백질의 SDS-PAGE
 적 요
 사 사
 인용문헌(REFERENCES)
저자
  • 박세준( 국립한경대학교 식물생태화학연구소 ) | Sei Joon Park ( Institute of Ecological Phytochemistry, Hankyong National University, Ansung 456-749, Korea. )
  • 박준영( 국립한경대학교 식물생태화학연구소 ) | Jun Young Park ( Institute of Ecological Phytochemistry, Hankyong National University, Ansung 456-749, Korea. )
  • 황수민( 국립한경대학교 식물생명환경과학과 ) | Su Min Hwang ( Department of Plant Life and Environmental Science, Hankyong National University, Ansung 456-749, Korea. )
  • 서명철( 농촌진흥청 국립식량과학원 작물환경과 ) | Myung Chul Seo ( Crop Environment research division, NICS, RDA, Suwon, 441-857, Korea )
  • 김태완( 국립한경대학교 식물생명환경과학과 ) | Tae Wan Kim ( Department of Plant Life and Environmental Science, Hankyong National University, Ansung 456-749, Korea. ) Corresponding author