논문 상세보기
The aim of the present study was to assess the effects of ammonia on physiological responses in Korean rockfish (Sebastes schlegeli). Interestingly, no mortality were observed when the specimens (301.1±8.0 g) were exposed to five levels of un-ionized ammonia (NH3) (control, 1, 2, 4, 8 mg L-1) for 3 hours. Furthermore, a significantly higher increase in gill Na+/K+-ATPase (NKA) pump activity with was detected due to the ammonia exposure. The activity of the fishes were found to be 4 and 8 mg L-1 NH3, which was significantly high compared to normal 1 mg L-1 NH3. Although ammonia exposure had no effect on plasma Cl-, exposure to both 4 and 8 mg L-1 NH3, however it led to increase in the plasma Na+, K+ and osmolality levels. Also, prolong ammonia exposure cause increase of plasma cortisol and glucose levels. The increase in glucose was accompanied by an increase in cortisol. The fish exposed to 4 and 8 mg L-1 NH3 showed significantly higher hematocrit than control group than those exposed to 1 and 2 mg L-1 NH3. The intensity of cell damage increased with the increase concentration and exposure to ammonia. Furthermore, hyperplasia, separation and epithelial necrosis were also observed in gill tissues. Taken together, the results showed that direction of changes to the investigated parameters can be used to determine the physiological responses of Korean rockfish to ammonia.
재료 및 방법
1. 실험어 및 암모니아 노출
2. 혈액 및 조직 샘플링
3. 혈액 분석
4. Na+/K+-ATPase (NKA) 활성 분석
5. 아가미의 조직학적 관찰
6. 통계 분석
결 과
1. 혈장 암모니아 농도
2. 아가미 NKA 활성
3. 혈장 Na+, K+, Cl- 및 삼투질농도
4. 혈장 코티졸 및 글루코스 농도
5. Ht
고 찰
적 요
사 사
REFERENCES
