Bioethanol was produced from Laminaria japonica hydrolystaes by sequential acidic (0.108 N HCl)/distilled water and enzymatic hydrolysis (Celluclast® 1.5 L) using Saccharomyces coreanus immobilized into/on aluminum silicate. Reducing sugar were hydrolyzed 140.5 and 122.7 mg/g-dry biomass under a acidic-enzymatic condition and a distilled waterenzymatic condition, respectively. In addition, the 8 repetition batch fermentations were carried out with the immobilized S. coreanus to verify the advantage of immobilization cell. As a result, we can obtain the ethanol of 12.1 ~ 24.3 mg/gdry biomass, and reuse the support, aluminium silicate, for 8 repetition batch fermentations without any breakdown.

• 정승미(목포해양대학교 환경⋅생명공학과) | Seung-Mi Jeong
• 황다혜(목포해양대학교 환경⋅생명공학과) | Da-Hye Hwang
• 박은영(서울시립대학교) | Eun-Young Park
• 김용진(목포해양대학교 환경⋅생명공학과) | Yong-Jin Kim Corresponding author