We provide the first report on Stigmaeopsis miscanthi Saito, which was identified from Chinese silver grass Miscanthus sinensis on Ulleung Island in Korea. This species is one of the S. celarius complex, which involves several cryptic species. S. miscanthi has longer second dorsal propodosomal setae (P2), which is an important characteristic for species identification in the genus Stigmaeopsis. We determined nucleotide sequences of the internal transcribed spacer 2 (ITS2) and cytochrome c oxidase subunit I (COI) of S. miscanthi. The COI sequence differed by 0.6% between Korean and Japanese strains. The comparison between S. miscanthi and S. celarius showed that ITS2 and COI differed by 7.2% and 7.9%, respectively. In addition, species-specific primer sets of both species were designed to show the species classification within the genus.