Silk fibroin can be damaged or degraded during degumming process. Therefore, it is expected that different structure and properties of silk fibroin can be obtained by different degumming method. However, effect of degumming method on the structure and properties of regenerated silk has not been considered, yet. In this study, Effect of degumming method on the structure and properties of solution, film, and electrospun fiber of regenerated silk was examined. Order of viscosity of regenerated silk solution as follows : Urea method > Acid method, HTHP method > Soap/soda method, Soda method. This viscosity difference among the degumming method strongly influenced the electro-spinning performance of regenerated silk perpared from different degumming method. Also, solution turbidity, crystallinity index (from FTIR), mechanical properties of silk were remarkably affected by degumming method.