The genus Panonychus has been reported only two species, P. ulmi and P. citri, in Korea. Two new species, P. mori Yokoyama, 1929 and P. caglei Mellot, 1968 were firstly identified from jujube orchards in Gyeongsan and kudzu vine in Byunsan peninsula in Korea. Morphological differences among four species have been described especially in aedeagus shape. Comparison of nucleotide sequences of both the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal RNA gene and the mitochondrial cytochrome oxidase subunit I (COI) gene were compared between four species. Phylogenetic analysis of ITS2 and COI sequences using neighbor-joining method showed that P. mori and P. caglei were most similar to each other and more closely related to P. ulmi than P. citri. In addition, species-specific primer sets of each species were designed based on ITS2 sequencesand can be used to diagnose species in this genus.