The blueberry gall midge, Dasineura oxycoccana (Johnson), which has been recently introduced into Korea, is a notorious pest on blueberries. This invasive insect has rapidly spread throughout Korea including Jeju island. So far, we have no epidemic information, such as invasion routes and subsequent dispersal rates in Korea. To understand population genetics of D. oxycoccana, we have developed 12 novel microsatellite loci. To obtain its sequence data, the next generation sequencing was performed using mixed individuals collected from Korea and USA. The developed loci were polymorphic, with 6 to 16 alleles in 35 individuals from a single population of Hwaseong. The analyses revealed that all 35 individuals had different multilocus genotypes with heterozygosity ranging from 0.568 to 0.790. These markers will facilitate population genetic studies of D. oxycoccana.

• Hyojoong Kim(Department of Biology, Kunsan National University, Gunsan, 573-701)
• Sangwook Park(Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921)
• Taek-Jun Kang(National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 441-440)
• Sejin Kim(National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 441-440)
• Heung-Sik Lee(Plant Quarantine Technology Center, Animal, Plant and Fisheries Quarantine and Inspection Agency, Suwon 443-400)