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Electron beam sensitivity and DNA damage in imidacloprid-resistant and -susceptible strains of Aphis gossypii
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/288067
The effect of electron beam irradiation on development and reproduction of imidacloprid-resistant (R strain) and -susceptible (S strain) Aphis gossypii were compared. Nymphs and adults of R and S strain were irradiated at target doses of 50, 100, 150, and 200 Gy. When nymphs were irradiated, emergence was not affected at all target dose, but number of F1 nymph was perfectly inhibited at 150 Gy in both strains. When irradiated to the adults, longevity slightly decrease at 150 Gy and above. Fecundity was strongly decreased at 100 Gy, but was not completely inhibited even at 200 Gy. Emergence of F1 nymph was decreased at 100 Gy and completely inhibited at 200 Gy. However, there were no significant differences on development and reproduction of R and S strain. We also conducted the comet assay immediately after irradiation and over the following 10 day period. Severe DNA fragmentation in A. gossypii cells was observed just after irradiation and the damage was repaired during the post-irradiation period in a time-dependent manner. These results suggest that electron beam irradiation induced abnormal development, reproduction, and DNA damage in A. gossypii, but there were no significant differences between R and S strain.
