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Comparative Whole Genome Transcriptome Analysis of Rice Stripe Virus Viruliferous and Non Viruliferous Small Brown Planthopper, Laodelphax striatellus

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  • URLhttps://db.koreascholar.com/Article/Detail/288085
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice and mediated by small brown planthopper, Laodalphax striatellus. So far, the studies have been mainly focused on the interaction between the host plant and the virus. In this study, for better comprehension of the interactions among Rice stripe virus, rice and small brown planthopper, transcriptomes of the RSV-viruliferous (RVLS) and non-viruliferous L. striatellus (NVLS) were comparatively analysed. For this, non-viruliferous L. striatellus were collected from non-infected rice field and fed RSV-infected rice for 5 days. With the RNAs prepared from the RSV-viruliferous and the non-viruliferous small brown planthoppers, we conducted Illumina RNA sequencing (Hiseq 2000) and then two transcriptome databases were generated from RVLS and NVLS, respectively. The transcriptome of RVLS and NVLS were campared to figure out how the gene expression of the insects affected by Rice Stripe Virus. RSV-dependently regulated genes analysed from this study may have important functions in the transmission and replication of RSV.

저자
  • Saes Byeol An(Department of Agricultural Biotechnology, Seoul National University)
  • Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Seok Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
  • Ying Fang(Department of Agricultural Biotechnology, Seoul National University)
  • Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
  • Seung Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
  • Shin Sang Woon(Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Woo Jin Kim(Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)