Sacbrood virus (SBV) is one of the most destructive honey bee virus. The virus causes failure to pupate and kills honey bee larvae. The infacted larvae`s color is change to brown. At the end, honey bee colony is destructed. Recently Korean Scabrood virus(KSBV) caused a great loss of Korean honey bee(Apis cerena) colonies for short period. Therefore, We need a highly rapid diagnosis method for rapid detection of KSBV.
In this study, We need amicro-scale chip-based real-time PCR system (GeneChecker®). This system was developed for rapid, specific PCR based diagnosis. This system has uncommonly fast heating and cooling system. So We was able to detecting of KSBV in Apis cerena in short time. This system needs small reaction volume(total 10ul). This volume include SsoFast™ Evagreen Supermix and serially diluted cDNA templates showed a high sensitivity of 101copies.That machine can setting each PCR stage time. A specific detection primer set (KSBV-123-F/R) was used to amplify a unique 123bp DNA fragment.
This PCR assays using serially diluted cDNA templates showed a high sensitivity of 101 copies. When applied to KSBV-positve samples, the result showed high specifity. The minimum diagnosis time was 9m 47s (30cycle). The amplied positive samples appear red fluorescent color.
This novel detection method could be used a PCR-based diagnositic tool (GeneChecker®). The results showed high sensitivity and specifity in short time. And this diagnosis method is expected to be applied to rapidly detect various pathogens.

• Nam-Hee Kim(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Mi-sun Yoo(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Young-Ha Kim(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Kondreddy Eswar Reddy(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Ha-Na Jung(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Suk-Chan Jung(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Seung-won Kang(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)