Virus infections of the honeybee(Apis mellifera) have been increasingly investigated during the last decade. In general, honeybee viruses are widespread and most of them persist as inapparent infections. We screened honeybee colonies for the presence of several bee viruses, including deformed wing virus(DWV), black queen virus(BQCV), Kashmir bee virus(KBV), Israeli acute paralysis virus (IAPV), sacbrood virus(SBV), acute bee paralysis virus(ABPV), using uniplex RT-PCR. Frequently simultaneous infections with different viruses are diagnosed in seemingly healthy bee colonies. Therefore we developed a multiplex RT-PCR assay for the simultaneous detection of multiple bee viruses.

• Ha-Na Jung(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Mi-Sun Yoo(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Young-Ha Kim(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Jin-Hyeong Noh(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Nam-Hee Kim(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Kondreddy Eswar Reddy(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Suk-Chan Jung(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)
• Seung-Won Kang(Center for Honeybee Disease Control, Parasite and Insect Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency.)