논문 상세보기

Production of BmCecB1 antimicrobial peptide in the transgenic silkworm

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/288430
모든 회원에게 무료로 제공됩니다.
한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

BmCecB1 are antimicrobial peptides from Bombyx mori and belongs to cecropin family. Antimicrobial peptides are important components of the innate immune systems in all living organism. This peptide has antibacterial activity against several Gram-positive and Gram-negative bacteria. To produce the BmCecB1 antimicrobial peptide, we constructed transgenic silkworm that expressed BmCecB1 gene under the control BmA3 promoter using piggyBac vector. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor vector and helper vector were micro-injected into 600 eggs of bivoltin silkworms, Baegokjam. In total, 49 larvae (G0) were hatched and allowed to develop into moths. The resulting G1 generation consisted of 22 broods, and we selected 2 broods containing at least 1 EGFP-positive embryo. The rate of successful transgenesis for the G1 broods was 11%. We identified 9 EGFP-positive G1 moths and these were backcrossed with wild-type moths. With the aim of identifying a BmCecB1 as antimicrobial peptide, we investigated the Radical diffusion Assay (RDA) and then demonstrated that BmCecB1 possesses high antibacterial activities against Gram-negative bacteria.

저자
  • Hye Lim Yeo(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Seong Wan Kim(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Seon Young Kim(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Eun Young Yun(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Kwang-Ho Choi(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Seong Ryul Kim(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Seok Woo Kang(Department of Agricultural Biology, National Academy of Agricultural Science)
  • Seung Won Park(Department of Biotechnology, Catholic University of Daegu,)
  • Tae Won Goo(Department of Agricultural Biology, National Academy of Agricultural Science)