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Characterization of Diadegma fenestrale Ichnovirus (DfIV) and Comparisons of its Gene Expression Patterns in Two Lepidopteran Hosts

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  • URLhttps://db.koreascholar.com/Article/Detail/288529
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The Diadegma fenestrale is known as parasitoid on potato tuber moth, Phthorimaea operculella and diamondback moth, Plutella xylostella. The Diadegma genus is reported to have symbiotic virus, ichnovirus, D. fenestrale Ichnovirus (DfIV) was identified from this species which is a first report. DfIV showed typical ichnovirus shape with two membranes surrounding the virus capsid. To identify DfIV genes, whole genome sequencing based on GS-FLX was conducted using purified total DfIV genomic DNA extracted from D. fenestrale calyx. About sixty ORFs were analyzed and several typical ichnovirus gene families were detected such as cys-motif, repeat element, vinnexin and vankyrin. Present study was focused on the gene expression patterns in two different lepidopteran hosts.

저자
  • Ju Il Kim(Highland Agriculture Research Center, NICS, RDA)
  • Min Kwon(Highland Agriculture Research Center, NICS, RDA)
  • Si Hyeock Lee(Department of Agricultural Biotechnology, Seoul National University)
  • Yong Gyun Kim(Department of Bioresource Sciences, Andong National University)
  • Jin Kyung Choi(Department of Life Sciences, Yeungnam National University)
  • Jong Wook Lee(Department of Life Sciences, Yeungnam National University)