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DNA Barcoding of Bradysia (Diptera: Sciaridae) for the Identification of Larval Stages on Agriculture Plants.
Many species of the genus Bradysia larvae are known as agriculture pest, because they are feed on stem or root of agriculture plants. Even though the damages are made by larval stages, the immature stages are hardly identified morphologically. The partial sequences of mitochondrial cytochrome c oxidase subunit I (COI) gene are constructed for 25 species of Bradysia with the identified voucher specimens. Comparing these data, Bradysia procera, and B. sp.1 (tilicola Group) could be identified from the larvae specimens by molecular COI universal region. Bradysia atracornea, B. nomica, and B. difformis are also confirmed from the larval samples in greenhouse crops, chinese cabbage, onion, and scallion etc. The mean Kimura two-parameter (K2P) interspecific divergence of genus Bradysia was 16.78%, and mean K2P intraspecific divergence was 0.52%. DNA
