The toxicity of red pine needle hydrodistillate (RPN-HD), 19 RPN-HD constituents and 12 structurally related compounds and control efficacy of four experimental spray formulations containing RPN-HD (0.5. 1, 2 and 3% sprays) to adult Dermatophagoides farinae was evaluated. RPN-HD (24 h LC50,68.33 μg cm–2) was toxic. Menthol was the most toxic compound (12.69 μg cm–2) and the toxicity of this compound and benzyl benzoate did not differ significantly from each other. High toxicity was also produced by α-terpineol, bornyl acetate, geranyl acetate, thymol, linalyl acetate, terpinyl acetate, citral, linalool and camphor (18.79–36.51 μg cm–2). These compounds were more toxic than either deet or dibutyl phthalate. In vapour-phase mortality tests, these compounds were consistently more toxic in closed versus open containers, indicating that their mode of delivery was largely a result of vapour action. RPN-HD 3% experimental spray provided 95% mortality against adult D. farinae, whereas permethrin (cis:trans,25:75)2.5gL–1 spray treatment resulted in 0% mortality. In the light of global efforts to reduce the level of highly toxic synthetic acaricides in indoor environments, RPN-HD and the compounds described merit further study as potential biocides for the control of Dermatophagoides populations as fumigants with contact action.

• Ju-Hee Lee(WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
• Jun-Ran Kim(WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Research Institute for Agriculture and Life Science, Seoul National University)
• Young Yull Koh(Department of Pediatrics, College of Medicine, Seoul National University)
• Young-Joon Ahn(WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)