The genus Pratylenchus is considered as one of the most important plant-parasitic nematodes around the world because of its broad spectrum host ranges and worldwide distribution. 72 species of root-lesion nematodes have already been described from various parts of the globe. Among all of them, only 5 species are regulated as a target species in quarantine inspection. As the importation of target items inspecting nematodes are surely increasing every year, detection rate of nematodes in quarantine also increased. However, the rate of species identification has been decreased due to lack of related information. The species belonging to genus Pratylenchus are shown very low identification rate because we have specimens and information about only 10 domestic species and 5 regulated species. In this study, we identified the species in the genus Pratylenchus and obtained related information for application in the quarantine field. Species identification was performed with extracted nematodes from plants obtained in the recent quarantine inspection. Newly identified species were 5 species including Pratylenchus crenatus, P. fallax, P. kumamotoensis, P. panamaensis and P. penetrans. The photos, measured value and morphological characters of each species are provided.