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Cloning of a scavenger receptor gene from Spodoptera exigua hemocytes
Scavenger receptors (SRs) are transmembrane cell surface molecules recognized in apophotic cells, bacteria and lipopolysaccharide. With no physiological information on SRs in insects except SR-CI of Drosophila melanogaster, a putative SR gene was cloned and characterized in Spodoptera exigua. A partial S. exigua SR gene was obtained from hemocyte transcripts and exhibited high homology with type C. Its expression was confirmed in all developmental stages. Among different tissues, S. exigua SR was expressed highly in hemocytes. To confirm change in SR expression by infection, Escherichia coli was injected to fifth instar and RNA was extracted after 10 hours. SR expression in hemocytes of E. coli injected larva was not significantly different from the control but SR expression in fat body of E. coli injected larva was higher than the control. It is expected that SRs of S. exigua are related with immune responses against bacteria such as E. coli. To address its function, S. exigua SR expression was suppressed by double-stranded RNA (dsRNA).
