In order to achieve the optimized pest control, correct estimation of pest densities is a prerequisite to monitor pest damage and to provide efficient pest management plans. Parameters regarding diffusion (e.g., diffusion constant) and population size (e.g., growth rate) were estimated by using diffusion equation. The time series dispersal data of Whiteflies collected in greenhouse were used for modeling. Cross-correlation analysis was conducted to reveal the range and direction of pest population invasion. Sampling theory was further investigated regarding estimation of densities, and population dynamics of Whiteflies were discussed in two dimensions.