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Study on solid-state conditions and growth characteristics of Paecilomyces lilacinus HY-4

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  • URLhttps://db.koreascholar.com/Article/Detail/289377
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Paecilomyces lilacinus HY-4 is an entomopathogenic filamentous fungus that has exhibited insecticidal activity against Adoretus tenuimaculatus and Tetranychus urticae. Strain HY-4 has attracted a great deal of industrial concerns because this organism can be applicable as a potent bio-pesticide. In this study, we developed an optimal diphasic fermentation technique for HY-4 conidial production. The substrate prescription which was made up of hulls or brans was obtained by screening of agricultural products, and the conidial production could reach a minimum of 2.5×1010 conidia/g after solid fermentation for 12 days in a given condition. It was interesting to note that strain HY-4 was propitious to sporulate more efficiently and productively in liquid fermentation process in the presence of insect pupa. For the industrial application of the HY-4 spores, their pH and thermal stabilities were evaluated on SDA agar plate as well. Additionally, the germination rate of HY-4 spores was still above 90% even when they were stored for 5 months at ambient temperature.

저자
  • Zhen Ji(Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Han-Young Cho(Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Do Young Kim(Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Yi-Joon Kim(R&D Center, Insect Biotech Co., Ltd.)
  • Dong-Ha Shin(R&D Center, Insect Biotech Co., Ltd.)
  • Kwang-Hee Son(Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Ho-Yong Park(Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology)