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DNA barcode construction for the Forest Insect Pests based on the Insect Mitochondrial Genome Database (IMGD)

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  • URLhttps://db.koreascholar.com/Article/Detail/289574
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

DNA ‘barcoding’ has potential applications in insect pest monitoring and quarantine since large numbers of DNA sequences for insect species identification have been reported in recent years. However, the exact number of relevant COI sequences in public databases such as NCBI (http://www.ncbi.nlm.nih.gov/) is not readily available. The IMGD (Insect Mitochondrial Genome Database; http://www.imgd.org) contains 162,847 partially sequenced mitochondrial gene entries originated from 35,067 hexapod species and makes it possible to check whether DNA sequences have been previously reported or not for certain insect species. In this study, we applied the IMGD to establish baseline data for the forest pest insects in Korea, before constructing a DNA barcode system. Retrieving data from the IMGD, we recognized that DNA sequences were already available for 73 of 259 species known as forest pest insects in Korea. Most of the 73 species with DNA sequences are common pests worldwide but there maining 186 species are endemic to the Eastern Palearctic region. Based on these data, we are proceeding to construct a Korean Forest Insect Pest DNA barcode database.

저자
  • Wonhoon Lee(Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University)
  • Seunghwan Lee(Insect Biosystematics Laboratory, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University)