Field survey to estimate extent of damage to Reticulitermes species were conducted from 2009 through 2011 in the various locations of Korea. We discovered Reticulitermes species from three different locations of Jeju island. Partial COII sequences of mt DNA and morphological characters were collected and compared with previous reports. As result of comparative sequence analysis and taxonomic study, it was identified as Reticulitermes speratus kyushuensis Morimoto 1968 and this is first report of Reticulitermes speratus kyushuensis from Jeju island in Korea which can be used as estimation of population structure and tool of control strategy.