Strawberry, Fragaria ananassa Duchesne, is one of the important horticultural crops cultivated in greenhouses. Tetranychus urticae is one of major strawberry pests, and Neoseiulus californicus and Phytoseiulus persimilis have been used as biological control agents for control of T. urticae. The interactions between T. urticae and N. californicus and between T. urticae and P. persimilis were investigated to compare their control efficiency for T. urticae on a spatially-structured strawberry leaf disc area at different temperatures and on different treatments. The experimental arena was an array of leaf discs (3 cm diameter) placed upside down on a water-saturated cotton pad in an aluminum pan (17.4 x 21.5 cm). Twenty leaf discs (4 x 5) were placed adjacent each other for allowing dispersal of T. urticae and its predatory mites. The temperature conditions were 20, 25, and 30°C and there were six different treatments. The overall population densities of T. urticae were influenced by temperature (20, 25, and 30°C) in N. californicus treatment. In the same temperature condition, P.persimilis was more effective than N. californicus to control T. uritcae. Two predator systems were better than one predator systems to suppress the population density of T. urticae at 25°C. Some results of this study could be used to understand the spatial association of T. urticae and its predatory mites in greenhouse crops and fields.