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Insecticidal capacity of novel recombinant baculoviruses to Plutella xylostella and Spodoptera exigua larvae
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/290306
This study was conducted to investigate the insecticidal capacity of several recombinant baculoviruses to P. xylostella and S. exigua larvae. NeuroBactrus was constructed as follows: the cry1-5 of Bacillus thuringiensis 2385-1 was inserted into Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) genome by fusion of polyhedrin-cry1-5-polyhedrin under the control of polyhedrin gene promoter, and insect-specific neurotoxin from the scorpion Androctonus australis (AaIT) under the control of early promoter from Cotesia plutellae bracovirus was introduced by fusion of orf603 partial fragment in the opposite direction of polyhedrin gene, respectively. Other recombinant baculoviruses derived from the NeuroBactrus - NBt-DelA (deleted AaIT), NBt-Del5 (deleted cry1-5), and NBt-DelA5 (deleted AaIT and cry 1-5) - were manufactured in serial passages in vitro. The data were analyzed by SPSS. The value of LC50 was lower when P. xylostella larvae fed on cabbage coated with NeuroBactrus (4068.4) than when it fed on cabbage coated with AcMNPV (4.5x106). Survival time (ST50) of P. xylostella larvae (2.54days) was shorter when it fed on cabbage coated with NeuroBactrus than when it fed on cabbage coated with other recombinant baculoviruses (7.54days, 7.68days, and 8.26days) and AcMNPV (9.67days). S. exigua larvae presented the same results.
