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Biochemical and molecular diagnosis of Insecticide resistance conferred by esterase and MACE based mechanisms of the Myzus Persicae (Hemiptera: Aphididae)
The green peach aphid, Myzus persicae (Sulzer), is one of the most serious pest in cabbage cultivation. Field survey was carried out to know the insecticide resistance levels in five main cabbage cultivation regions (Pyeong-chang, Hong-cheon, Bong-wha, Mu-ju and Je-ju) in 2009. The green peach aphid can resist a wide range of insecticides in five surveyed local populations. Among the nine tested insecticides, four chemicals (methomyl, bifenthrin, pymetrozine and flonicarmid) showed less than 50% mortality in the recommended concentration in all populations. Multi resistant (MR) strain was selected from these populations and esterase over-expression, modified AChE (MACE) and mutation(s) in para-type sodium channel were analysed using native IEF and quantitative sequencing with five local populations. Esterase over-expression and MACE (StoF mutation) were observed in all populations including MR strain. LtoF mutation is well known as a kdr mutation in para-type sodium channel. However, even though LC50 values of MR strain noted over 2,000 times higher than that of susceptible strain against bifenthrin, any mutation was not detected in para type sodium channel and also local populations. These results suggested that unusual case could be existed in pyrethroid resistance mechanism in green peach aphid.
