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Sequence analysis and expression of three major glycoproteins of Aujeszky’s disease virus NYJ strain in Bm5 Cells

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  • URLhttps://db.koreascholar.com/Article/Detail/290433
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Aujeszky’s disease (AD), also called pseudorabies, is an infectious viral disease, caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. AD affects many countries and regions in the world, causing important economic losses, mainly due to international trade restrictions. In this study, to determine the characteristics of the Aujeszky’s disease virus (ADV), NYJ strain, which was isolated from the serum of an infected pig in 1987, we investigated the nucleotide sequence and expression of the glycoproteins gB, gC, and gD using the bBpGOZA system. We found that the glycoproteins gB, gC, and gD of NYJ consisted of 2751 bp, 1443 bp, and 1203 bp, respectively. Comparison of the NYJ with the other strains revealed nucleotide sequence identity ranging from 91.tito 99.0%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The NYJ strain was formed a distinct branch with high bootstrap support. The expression of glycoprotein gD in insect cells was characterized by SDS-PAGE and Western blotting with an anti-ADV polyclonal antibody. Glycoprotein gD of approximately 45 kDa was detected. The results of this study have implications for both the taxonomy of ADV and vaccine development.

저자
  • Hyun Na Koo(Department of Agricultural Biology, Chungbuk National University)
  • Jeong Mi Oh(Department of Agricultural Biology, Chungbuk National University)
  • Jae Young Choi(School of Agricultural Biotechnology, Seoul National University, Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Kwang Sik Lee(College of Natural Resources and Life Science, Dong-A University)
  • Jong Yul Roh(School of Agricultural Biotechnology, Seoul National University)
  • Yeon Ho Je(School of Agricultural Biotechnology, Seoul National University)
  • Byung Rae Jin(College of Natural Resources and Life Science, Dong-A University)
  • Sung Sik Yoo(Choong-Ang Vaccine Laboratory)
  • Jae Su Kim(AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd.)
  • Young In Kim(AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd.)
  • In Joong Yoon(Choong-Ang Vaccine Laboratory)
  • Soo Dong Woo(Department of Agricultural Biology, Chungbuk National University)